Antioxidant Level of Sodium Glutamate in Wister Rat whole Brain, Liver and Kidney from Thevetia peruviana kernel

Abstract

The researchers investigated the antioxidant levels of sodium glutamate produced from Thevetia peruviana kernel in the whole brain, liver, and kidney of Wistar rats. The Thevetia peruviana kernels used in this study were collected from Lafe area, Akure, Ondo State, Nigeria, and identified at the Department of Crop, Pest, and Soil Management, Federal University of Technology, Akure. The samples were processed using three drying techniques: oven-dried, air-dried, and sun-dried. The processed kernels were ground into powder, stored in airtight plastic containers, and preserved in a refrigerator at 4°C before analysis. Sodium glutamate production was conducted, with the oven-dried samples yielding the best sodium glutamate quantity, which was subsequently used for further analysis. Twenty Wistar rats were purchased and grouped by weight. The groups (n = 4) were divided into five baskets (1–5). The first basket served as the control group (0 mg/kg) and received no sodium glutamate, while the remaining four baskets were treated with different dosages of sodium glutamate at 1 mg/kg, 10 mg/kg, 100 mg/kg, and 1000 mg/kg, respectively. The antioxidant levels, specifically Reduced Glutathione (GSH) activity, were measured in the brain, liver, and kidney tissues of the Wistar rats. Results indicated that the sodium glutamate produced (seasoning) was safe and showed promise as an intervention in managing and preventing oxidative stress, primarily due to its antioxidative protective properties. The highest dosage administered (1000 mg/kg) demonstrated a tolerable, non-toxic effect on the organs studied.